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Objective To evaluate the mean blood flow(MF) and pulsatility index(PI) of individual and sequential sa-phenous vein grafts in posterior descending artery(PDA) in the same patient undergone off-pump coronary artery bypass grafting (OPCABG).Methods 140 patients with coronary artery disease were studied.Sequential surgical technique using single large saphenous vein was used in grafts of diagnostic artery(DIAG)(side-side), obtuse marginal artery(OM)(side-side), and PDA( end-side) .Bulldog clamps were used to temporarily arrest the anastomoses of DIAG and OM near the large saphenous vein .Thus the sequential graft became to the single graft , the MF and PI of PDA were recorded in the simulating single graft by the transit-time flow measurement(TTFM), after that, bulldog clamps were removed and the MF and PI of PDA were recorded again in the real sequential graft.The data were compared.Six months later after surgery, coronary CTA were done in all pa-tients in clinic.Results The MF of single graft and sequential graft was(22.5 ±13.1)ml/min and(22.2 ±12.9)ml/min(P>0.05).The PI of single graft and sequential graft was 2.43 ±0.94 and 2.38 ±0.88(P>0.05).The patency of all grafts is good in all patients after 6 months follows-up.Conclusion There ars no hemodynamic differences between individual and sequential grafts in PDA.It is reasonable that the short-term surgical result is the same in individual and sequential grafts in PDA.
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Objective To observe the value of speckle-tracking imaging (STI) in evaluation on degree of coronary artery stenosis in patients with coronary artery disease (CAD).Methods Totally 74 CAD patients,including 59 with coronary artery stenosis (coronary artery stenosis group) and 15 without coronary artery stenosis (no coronary artery stenosis group) underwent STI and echocardiography.Based on Gensini scores,the patients in coronary artery stenosis group were further divided into mild,moderate and severe subgroups.The average global longitudinal strain (GLS-Avg),basement global longitudinal strain (GLS-Bas),middle global longitudinal strain (GLS-Mid) and apical global longitudinal strain (GLS-AP) value were measured and compared.Results GLS-Avg,GLS-Bas,GLS-Mid and GLS-AP value in coronary artery stenosis group were lower than those in no coronary artery stenosis group (all P ＜ 0.001).In coronary artery stenosis group,with the increase of stenosis severity,GLS-Avg,GLS-Bas,GLS-Mid and GLS-AP value decreased,and statistical differences were found between each two subgroups (all P＜0.05).In coronary artery stenosis group,there were positive correlations between GLS-Avg,GLS-Bas,GLS-Mid,GLS-AP value and Gensini scores (r=0.861,0.847,0.819 and 0.778,all P＜ 0.05).Conclusion GLS value of STI can reflect the severity of coronary artery stenosis in CAD patients.
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Objective To explore the effect of a Chinese Medicine Xionggui Liujunzi Decoction on the experimental coronary heart disease in rats. Methods Rats were randomly divided into five groups: the blank control group, the model control group, the western medicine control group (simvastatin), the Chinese medicine control group ( compound Danshen dripping pills) and the Xionggui Liujunzi Decoction group. The coronary heart disease was induced by intragastric gavage of fat emulsion (10 mL/kg, q. d. for 12 weeks) and pituitrin (30 U/kg, q. d. for 3 days) was intraperitoneally injected to induce coronary artery spasm. Changes of the ST segment in electrocardiogram (ECG) and the blood lipids were detected, and the levels of the inflammatory factors including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), highly sensitive C-reactive protein (hs-CRP), monocyte chemotactic protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1), the vascular endothelial active substances including ET-1, NO, TXA2 and PGI2, and the indicators of fibrinolytic system function such as PAI-1, t-PA in the plasma were measured. Results Compared with the model control group, each drug treatment showed better effects on the ST segment of the electrocardiogram, blood lipids, levels of the inflammatory factors and vascular endothelial active substances, and the fibrinolytic function, with significant differences (P ＜ 0. 05). In particular, Xionggui Liujunzi Decoction has a significantly better effect than the compound Danshen dripping pills and simvastatin (P＜ 0. 05). Conclusions Xionggui Liujunzi Decoction can improve the ST segment of the electrocardiogram, blood lipids, levels of the vascular endothelial active substances and fibrinolytic function of the rat model of coronary heart disease, and alleviate inflammation responses, showing a significant effect on coronary arteriosclerosis and myocardial ischemia in rats.
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OBJECTIVES@#To explore the value of mast cell tryptase and brain natriuretic peptide（BNP） in the differential diagnostic of sudden death due to hypersensitivity and coronary atherosclerotic heart disease.@*METHODS@#Totally 30 myocardial samples were collected from the autopsy cases in the Department of Forensic Pathology, Shanxi Medical University during 2010-2015. All samples were divided into three groups： death of craniocerebral injury group, sudden death of hypersensitivity group and sudden death of coronary atherosclerotic heart disease group, 10 cases in each group. Mast cell tryptase and BNP in myocardium were detected by immunofluorescence staining and Western Blotting.@*RESULTS@#Immunofluorescence staining showed that the positive staining mast cell tryptase appeared in myocardium of sudden death of hypersensitivity group and coronary atherosclerotic heart disease group. Among the three groups, the expression of mast cell tryptase showed significantly differences through pairwise comparison （P<0.05）; The expression level of BNP in sudden death of coronary atherosclerotic heart disease group were significantly higher than the sudden death of hypersensitivity group and death of craniocerebral injury group （P<0.05）. The difference of the expression level of BNP between the sudden death of hypersensitivity group and the death of craniocerebral injury group had no statistical significance （P>0.05）.@*CONCLUSIONS@#The combined detection of the mast cell tryptase and BNP in myocardium is expected to provide help for the forensic differential diagnosis of sudden death due to hypersensitivity and coronary atherosclerotic heart disease.
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Humans , Male , Anaphylaxis , Autopsy , Blotting, Western , Case-Control Studies , Coronary Artery Disease/complications , Death, Sudden, Cardiac/etiology , Diagnosis, Differential , Fluorescent Antibody Technique , Forensic Pathology , Myocardial Infarction , Myocardium/metabolism , Natriuretic Peptide, Brain/metabolism , Tryptases/metabolismABSTRACT
Objective To observe the expression of phosphatase and tensin hom ology deleted on chrom o-som e ten (PTEN) in m yocardial tissue in patients w ith coronary heart disease, and explore the relevance betw een the expression of PTEN and the occurrence and developm ent of coronary heart disease. Methods A total of 16 death cases w ith pathological diagnosis of coronary heart disease w ere collected as experi-m ental group, and 19 cases w ithout m yocardial lesions w ere selected as control group. The expression of PTENprotein and its m RNA w ere detected by im m unohistochem istry and real-tim e fluorescence quanti-tative PC R respectively. The correlation betw een the expression of PTEN and the pathogenesis of coronary heart disease w as analyzed. Results The expression of PTENprotein in myocardium in cases w ith coro-nary heart disease w as significantly low er com pared w ith the control group (P0.05). Conclusion PTEN m ay be involved in the occurrence and developm ent of coronary heart disease.
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Objective To explore the value of mast cell tryptase and brain natriuretic peptide(BNP)in the differential diagnostic of sudden death due to hypersensitivity and coronary atherosclerotic heart dis-ease.Methods Totally 30 myocardial samples were collected from the autopsy cases in the Department of Forensic Pathology, Shanxi Medical University during 2010—2015. All samples were divided into three groups:death of craniocerebral injury group, sudden death of hypersensitivity group and sudden death of coronary atherosclerotic heart disease group, 10 cases in each group. Mast cell tryptase and BNP in myocardium were detected by immunofluorescence staining and Western Blotting.Results Immunofluo-rescence staining showed that the positive staining mast cell tryptase appeared in myocardium of sudden death of hypersensitivity group and coronary atherosclerotic heart disease group. Among the three groups, the expression of mast cell tryptase showed significantly differences through pairwise comparison(P0.05).Conclusion The combined detection of the mast cell tryptase and BNP in myocardium is expected to provide help for the forensic differential diagnosis of sudden death due to hypersensitivity and coronary atherosclerotic heart disease.
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Arteriosclerosis is a chronic inflammatory vascular pathology that features a leading cause of coronary artery disease contributing to significant mortality and reduced quality of life. The recent identification of the possible role of infections in the initiation of a serious of inflammatory events represents an interesting development towards the better understanding of immune mediated vascular injury and premature atherosclerosis in patients with chronic HCV infection. A number of factors related to chronic HCV infection have been hypothesized to contribute to arteriosclerosis. The current review displays some of the aspects of interaction between the chronic viral infection, the immune system and cytokine networks and its relation to the increased risk of coronary artery disease.
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Objective: To explore influence of sleep deprivation (SDP) on serum interleukin-1β (IL-1β) level in rats with coronary atherosclerosis (CAS). Methods: A total of 96 healthy SD rats were randomly and equally divided into normal control group, CAS group, SDP group and SDP + CAS group according to number table; rats in CAS group and SDP + CAS group were fed with high cholesterol diet to establish CAS animal model; SDP group and SDP + CAS group received SDP through small platform with water environment. Radioimmunoassay was used to measure serum concentration of IL-1β; and serum concentrations of total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were measured respectively; above indexes were compared among four groups. Results: (1) Compared with serum IL-1β level of normal control group, there were significant increase in SDP group, CAS group and SDP + CAS group[(13.33±3.20) ng/L vs. (14.40±4.41) ng/L vs. (22.21±5.94) ng/L vs. (30.17±7.45) ng/L] (P<0.05～<0.01); (2) Levels of TC, TG and LDL-C in CAS group and SDP + CAS group were significantly higher, level of HDL were significantly less than those of normal control group and SDP group (P<0.05～<0.01), levels of above indexes in SDP + CAS group were significantly higher or less than those of CAS group (P<0.05 all). Conclusion: Sleep deprivation can significantly heighten serum interleukin-1β level and total cholesterol, triglyceride, low density lipoprotein-cholesterol levels, decrease HDL level in rats with coronary atherosclerosis or sleep deprivation.
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Objective Off-pump coronary artery bypass (OPCAB),minimally invasive direct coronary artery bypass (MIDCAB) and robotic-assisted coronary artery bypass (RA-CAB) are all used to treat isolated left anterior descending artery (LAD) disease.The aim of this study is to compare the early outcomes after these three procedures.Methods From February 2009 to May 2012,102 consecutive patients underwent revascularization of LAD.31 patients were treated by OPCAB,45 by MIDCAB and 26 by RA-CAB.Patients received sternotomy in the OPCAB procedures.The MIDCAB procedures were performed through a 10-cm anterolateral muscle-sparing minithoracotomy.In the RA-CAB procedures,left internal mammary arteries (LIMA) were harvested with the aid of da Vinci surgical system and sewing of the anastomoses was performed under direct vision by a 3-cm anterolateral minithoracotomy.Results No significant difference was observed in graft flow,pulse index,renal failure,reoperation for hleeding,new onset of arterial fibrillation and deep wound infection between these three groups.There was also no significant difference in peri-operative mortality,major adverse cerebro-cardiovascular events (MACCE) between these three groups.Compared with OPCAB,MIDCAB and RA-CAB significantly reduced the need of blood transfusion (4.4％ vs.32.3％,P＜ 0.05; 7.7％ vs.32.3％,P＜0.05).The patients receiving RA-CAB had shorter length of postoperative stay than whom receiving OPCAB[(8.8 ± 3.2) days vs.(12.4 ± 7.7) days,P ＜ 0.05)].There is no significant difference between the outcomes of MIDCAB and RA-CAB.Conclusion These findings indicated that MIDCAB and RA-CABwere feasible,effective and safe options for revascularization of isolated LAD disease.MIDCAB and RA-CAB showed the advantage of less invasive and faster recovery,compared with OPCAB.Therefore,MIDCAB and RA-CAB should be the routine treatment for patients with isolated LAD disease.In some advanced centers,RA-CAB will be the preferred method.The mid-and long-term outcomes of these three methods should be further investigated.
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Objective Analysis of myocardial microvascular perfusion in patients with chronic total coronary occlusion (CTO) who underwent a coronary artery bypass graft (CABG) use real-time myocardial contrast echocardiography (RTMCE),to provide an effective method of detecting viable myocardium and a reference for the choice of CABG indications.Methods Twenty-seven patients with CTO underwent RTMCE 1 week before CABG,they underwent follow-up echocardiography and coronary artery 256-slice multislice computed tomography aagiography 1 year after CABG.Myocardial viability was defined as a postoperative ultrasound wall motion significantly improved ≥ 1 point.Semi-quantitative analysis of contrast images,myocardial viability was defined as myocardial perfusion score ≤ 2 points.Viable myocardium by quantitative assessment of myocardial blood flow (MBF) was determined by analyses of receiver-operating characteristic (ROC) curves.Results Patients with LVEF increased significantly after CABG (P ＜ 0.01),Of 259 segments with wall motion abnormality,149 (58％) showed wall motion significantly improved ≥ 1 point after CABG,considered viable myocardium,110 (42％) were not observed in wall motion improved,considered to be non-viable.The viable myocardial segments were significantly greater than non-viable myocardial segments in A,β,A × β value (P ＜ 0.01).Compared with the semi-quantitative analysis,quantitative analysis of MBF increased the sensitivity and accuracy of RTMCE for detecting viable myocardium (P ＜ 0.05).Conclusion RTMCE could accurately assess myocardial viability and provide a strong reference for clinical decision making and judging prognosis.
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Objective To evaluate the risk of hyperuricemia in patients younger than 45 with coronary artery disease.Methods Six hundred and seventy-seven patients with coronary artery disease under 45 years old at disease onset were recruited retrospectively.The subjects were divided into hyperuricemia group (n=164) and normal uric acid group (n=513).Patients with coronary artery disease with hyperuricemia were analyzed for clinical characteristics.The risk of hyperuricemia was analyzed on the severity of coronary lesions.T-test,x2 test and Logistic regression analysis were used for statistical analysis.Results Comparing with patients with normal uric acid,patients in the hyperuricemia group were male predominant and more prevalent with hypertension [197(38.5％) and 93(56.7％) ; x2=16.85,P＜0.01],hypedipidemia [274(53.4％) and 130 (80.7％); x2=37.58,P＜0.01],and cardiac dysfunction [24 (4.7％) and 17 (10.4％); x2=7.18,P=0.01].Hyperuricemia increased the risk of coronary artery disease complicated with cardiac dysfunction [ORlcorrection=2.66,95％CI (1.28,5.53)],and it also increased the risk of coronary artery disease concurrent with cardiac arrhythmia [OR2correction=1.28,95％CI (1.00,1.65)] revealed by multivariate regression analysis.Conclusion In young patients under 45 years old with coronary artery disease,hyperuricemia is the independent risk factor for coronary artery disease complicated with cardiac dysfunction and arrhythmia.
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Objective To explore the clinical value of combined detection of D-dimer,coagulation four indices and hs-CRP in diagnosis of coronary heart disease (CHD).Methods D-Dimer,coagulation four indices and hsCPR level were detected in 67 patients with CHD[22 stable angina(SA) pectoris patients,20 unstable angina (UA) pectoris patients and 25 acute myocardial infarction (AMI) patients] and 20 healthy people(normol control group).According to the result of coronary arteriongraphy,the patients with CHD were divided into single vessel disease group (n =21),double vessel disease group (n =23) and three vessel disease group (n =23).The levels of plasma D-D,fibrinogen(FIB) and hs-CRP were detected and compared.Results Compared with UA group,SA group,control group respectively,D-D,fibrinogen and hs-CRP level of AMI group had statistical significance (P ＜ 0.01) ;Compared with SA group,control group,D-D,fibrinogen and hs-CRP level of UA group had statistical significance (P ＜ 0.05) ;Compared with control group,hs-CRP level of SA group had statistical significance (P ＜ 0.01).The comparision of PT,APTT and TT level between each two groups,there was no statistical difference (P ＞ 0.05).The levels of plasma DD and FBG in single vessel disease group,double vessel disease group and three vessel disease group were significantly higher than those in NC group(P ＜ 0.05).The levels of plasma DD and FBG in three vessel disease group were significantly higher than those in single vessel disease group and double vessel disease group(P ＜ 0.05).The levels of plasma DD and FBG in double vessel disease group were significantly higher than those in single vessel disease group (P ＜ 0.05).The comparision of PT,APTT and TT level between each two groups,there was no statistical difference (P ＞ 0.05).Conclusion The level of D-D,FIB and hs-CRP have close relationship with the severe degree of CHD and can help clinical doctor to predict the type of CHD.
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Objective: To explore the relationship between insulin sensitivity index （ISI）and extent of coronary atherosclerosis in Uygur and Han nationality patients. Methods: A total of 125 patients with coronary heart disease were enrolled, including 59 Uygur nationality patients and 66 Han nationality patients. There were 42 cases with stable angina pectoris, 52 cases with unstable angina pectoris, 31 cases with old myocardial infarction, 53 cases with type 2 diabetes and 35 cases with hypertension. Relevant medical history of patients [age, body mass index (BMI) etc.] was collected and laboratory examination （blood lipid, fasting blood glucose, fasting insulin etc.）were performed, and extent of coronary atherosclerosis was evaluated by coronary angiography in all patients. ISI was calculated and its relationship with extent of coronary atherosclerosis was analyzed. Results: In both nationality patients, ISI was negatively correlated with extent of coronary atherosclerosis (Han nationality: r=-0.71, P=0.03, Uygur nationality: r=-0.52, P=0.04). ISI was negatively correlated with BMI, glycosylated hemoglobin (HbA1c) and low density lipoprotein –cholesterol (LDL-C) (r=-0.45, -0.68, -0.55, P<0.05 all) in Han nationality patients; ISI was positively correlated with high density lipoprotein–cholesterol (HDL-C, r=0.67, P<0.01), and negatively correlated with HbA1c, LDL and lipoprotein a (r=-0.47, -0.35, -0.42, P<0.05) in Uygur nationality patients. Conclusion: Insulin resistance participates and promotes formation and development of coronary atherosclerotic plaques. Controlling weight, blood glucose and LDL-C levels etc. can decrease insulin resistance and prevent occurrence and development of coronary heart disease.
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Objective To explore the changes of placental growth factor (PLGF) in patients with acute coronary syndrome (ACS).Methods One hundred and twelve patients were divided into four groups according to clinical data and coronary angiography:non-coronary artery disease (non-CAD) group (27 cases),stable angina (SA) group (28 cases ),unstable angina (UA) group (29 cases) and acute myocardial infarction (AMI) group (28 cases).Fifty-seven patients with ACS were divided into single lesion group (19 cases),twice lesion group ( 16 cases) and triple lesion group (22 cases) by coronary angiography,and 0-7 scores group (23 cases),8-15 scores group (27 cases) and 16-32 scores group (7 cases) by Jenkins scores.The serum PLGF levels of all the cases were determined on admission.The serum PLGF levels of 40 cases receiving percutaneous coronary intervention (PCI) were determined at 30 min before operation,the first and second day after operation.Results The serum PLGF levels of AMI group and UA group were (38.33 ±10.14) ng/L and (37.44 ± 13.32) ng/L respectively,which were significant higher than that of SA group [(20.90 ± 3.88) ng/L] and non-CAD group [(20.34 ±4.53) ng/L](P＜ 0.01),and there was no significant difference between AMI group and UA group,SA group and non-CAD group (P ＞0.05).There was no significant difference in the serum PLGF level among triple lesion group,twice lesion group and single lesion group (P ＞ 0.05 ).There was no significant difference in the serum PLGF level among 0-7 scores group,8-15scores group and 16-32 scores group (P ＞ 0.05).The serum PLGF level of patients receiving PCI on the first day after operation [ (32.03 ± 7.41 ) ng/L] was significantly higher than that before operation [ (23.86 ± 6.91 ) ng/L](P＜ 0.05 ),and on the second day after operation the serum PLGF level [ (29.37 ± 6.99) ng/L] was less than that on the first day after operation,showing a fall tendency(P＞ 0.05).Pearson correlation analysis showed that the serum PLGF levels in patients with ACS were not related with cardiac troponin T and creatine kinase isozyme-MB (r =-0.158,P=0.421;r =0.302,P=0.118).Conclusion The serum PLGF level of ACS patients is an inflammatory marker reflecting coronary atherosclerotic plaque destabilizing or plaque ruptured,and may also play an important role in early diagnosis of the borderline case of ACS.
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Objective Analysis the clinical data of high-EuroSCORE 114 patients due to ronary surgery to elucidatethe surgical advantages between on-pump and off-pump CABG.Methods From September 2008 to March 2011,114 highEuroSCORE patients due to coronary surgery were randomly divided into off-pump group 48,and on-pump gronp 66 cases.Preoperative,intra-operative and peri-operative clinical data of all the patients were collected.All patients were followed up for 1 month postoperatively.Results The baseline of the two groups had no significant difference.Application of internal mammary artery,positive inotropic drugs and IABP assistance between the two groups were similar,P ＞0.05,Compared with the offpump group,patients in on-pump gnup had longer operation time,hut more graft counts and a higher rate of revascularization [ (3.71±0.55)gnifis vs.(2.82±0.39)grafts ],P ＜ 0.05.There were no significant differences of peri-operative mortality and post-operative complications between the two groups,P ＞0.05.The patients in on-pump group had more post-operative chest drainage in the first 24 hours[ (875.0±134.2)ml vs.(589.4±102.5)ml] and blood transfusion[ (656.3±84.4)ml vs.(433.3±62.9) ml ] compared with the off-pump group,P ＜ 0.05.The data of l-month follow up denonstrated that echocardiographic data,NYHA and symptoms of angina of the two groups had no significant difference,P ＞ 0.05.Conclusion Compared with OPCAB,CCAB did not increase postoperative renal failure,neurological complications and lung injury in highrisk patients,but had more complete revascularization.The disadvantage of CCAB was postoperative bleeding,blood productsuse.
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Objective To evaluate the ability of further discriminating diagnosis of the headchest lead electrocardiogram (HCECG) in elderly patients with abnormal Q waves in routine lead electrocardiogram (RLECG) in inferior lead. Methods The 55 male patients, aged 65-88 years,with abnormal Q waves in both lead Ⅲ and aVF were selected and divided into two groups: myocardial infarction (MI) group and non-MI group, according to if the patient had a history of acute MI. All the patients accepted examination of coronary computed tomographic angiography (CTA) and ultrasound cardiogram, those with Wolff-Parkinson-White syndrome and myocardial hypertrophy were excluded.The 30 individuals of control group had no abnormal Q waves in lead Ⅱ , Ⅲ and aVF. HCECG and RLECG were recorded simultaneously in respective groups and occurrence rate of Q waves in correlative leads Ⅱ, Ⅲ, aVF and HL3, H0, HR3 were matched and compared, sensitivity and specificity were compared in respective leads. Results There were serious stenosis in 22 patients (100%) in MI group, and there were mild stenosis in 10 (30. 3%) and moderate stenosis in 23 patients (69.7%) in non-MI group. There was no significant difference between HCECG and RLECG in occurrence rate of Q waves in MI group (P＞ 0. 05 ). Non-MI group left anterior axillary line, Ⅱ ,Ⅲ, right anterior axillary line, near the anterior midline, aVF without Q wave and exclusion of old MI diagnostic specificity were 100%, 97.0%(32/33), 97.0% (32/33), 15.2% (5/33), 100%, 39.4%( 13/33)respectively. Conclusions Pseudo-changes are rarely found in HCECG and there is a higher degree of conformity in HCECG with coronary lesions, therefore HCECG may be used to discriminate whether the inferior abnormal Q waves occurred in RLECG are truly abnormal or not.
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Objective: To establish a real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) method for examining the expression of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1)gene in the peripheral blood mononuclear cells (PBMCs) in patients with coronary heart disease (CHD),so as to explore the relationship between Siglec-1 mRNA expression and the development/progression of CHD. Methods: Based on the fluorescent SYBR Green method, a real-time quantitative RT-PCR was set up. The expression of Siglec-1 gene in the PBMCs of 57 patients with CHD and 38 healthy controls were measured by ABI PRISM 7000 Sequence Detection Systems. The blood lipid levels were determined in all the subjects by routine biochemical examination. Results: The mean Siglec-1 mRNA copy was significantly higher in the CHD group than that in the healthy control group (mean 3.23 folds, ranging 1.28-8.11 folds, P < 0.01). The expression in the acute myocardial infarction group,stable angina and unstable angina group were 3.32(1.38-7.97),2.56(0.88-7.42),and 3.35(1.25-8.96) folds that of the control group,respectively. No significant difference was observed in the normalized Siglec-1 mRNA copy number between CHD group with normal level of serum lipids and abnormal level of serum lipids. Conclusion: A real-time quantitative RT-PCR method for detecting the expression of Siglec-1 gene in PBMCs has been successfully established. The expression of Siglec-1 is dramatically increased in PBMCs in CHD patients.
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Objective To investigate the association of osteoporosis and coronary artery calcification in elderly patients with type 2 diabetes mellitus (T2DM).Methods A total of 82 elderly T2DM patients underwent dual-energy x-ray absorptiometry scanning (DXA) of lumbar spine and femur neck for getting bone mineral density (BMD),and dual-source computed tomography (DSCT) of coronary artery for calculating calcification score and total calcification score (TCS).All subjects were divided into two groups:osteoporosis group and non-osteoporosis group.The levels of serum calcium (Ca),parathyrin (PTH),phosphorus (P),alkaline phosphatase (AKP),triglyceride (TG),total cholesterol (TC),high density lipoprotein-cholesterol (HDL-C),low density lipoproteincholesterol (LDL-C) and glycosylated hemoglobin (HbA1c) were detected.Results Compared with non-osteoporosis group,the levels of serum Ca,PTH and TCS were higher [(2.32± 0.15)mmol/L vs.(2.04±0.20) mmol/L;(5.64±1.97) pmol/L vs.(5.01±1.93) pmol/L;(374.4±433.5) scores vs.(242.5±224.8) scores,t=5.790,5.331 and 2.248,all P<0.05] in osteoporosis group.Correlation analysis showed TCS was negatively associated with BMD of L2-4 and femur neck,while was positively associated with serum Ca and PTH (r=0.310,0.246,0.290,0.284 and 0.324,0.575 all P<0.05).Conclusions Osteoporosis is associated with coronary atherosclerosis.TCS could be considered as an index for judging the relationship between osteoporosis and coronary atherosclerosis.
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Objective To investigate the activity of partial plasma coagulation factor activity and its clinical significance in occurrence of acute coronary syndrome(ACS). Methods The activities of co-agulation factors(FⅤ.C, FⅦ :C, FⅧ :C, FⅩ:C and FⅪ :C) were measured in 100 patients with acute myocardial infarction(AMI group) and 69 patients with unstable angina pectoris in acute stage(UAP group), the results were compared with those of 120 cases of stable angina pectoris(SAP group) and 80 healthy controls(heahhy control group). The relationship was analyzed between activities of coagu-lation factors and A MI as well as acute stage UAP. Results The patients with acute myocardial infarc-tion and unstable angina pectoris in acute phase had significantly higher plasma FⅤ.C, FⅦ :C, FⅧ :C, FⅩ:C and FⅪ :C compared with those of healthy and SAP subjects(P<0.05) ; there were no sig-nificant differences of the above activities between SAP group and healthy control group(P>0.05).Conclusion Significantly higher activation of FⅤ.C, FⅦ :C, FⅧ :C, FⅩ:C and FⅪ :C and a hyper-coagulabale state exist in acute coronary syndrome(ACS) patients in acute stage.
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Objective To evaluate the clinical diagnostic value of coronary artery calcification score(CACS)of multi-slice spiral computed tomography(MSCT)combined with carotid intima-media thickness(IMT)measure in elderly patients with coronary artery disease(CAD). Methods CACS of MSCT,carotid IMT measure,and coronary angiography were performed in 68 patients,including 36 cases with CAD(CAD group)diagnosed by coronary angiography and 32 cases(control group)with coronary arterial stenosis(＜50% stenosis).CACS and carotid IMT were compared between two groups. Results The coronary artery calcification score was significantly increased in CAD group compared with the control group[(349.5±86.3)vs.(74.7±25.2),t=13.670,P＜0.01],and it was increased with the severity of coronary arterial stenosis.The carotid intima-media thickness in CAD group showed significant difference with that in control group[(1.11±0.05)mm vs(0.69±0.13)mm,t=13.587,P＜0.01].In CAD group,CACS exhibited a significant positive correlation with carotid IMT(r=0.950,P＜0.01).The positive rates of CACS and carotid IMT were both 77.8% (28 cases)in CAD group and both 12.5%(4 cases)in control group,which showed significant difference between two groups(X2=28.976,P＜0.01). Conclusions CACS of MSCT combined with carotid IMT have high sensitivity and specificity in evaluating coronary arteriaI stenosis.It can be used as a non-invasive examination to diagnose CAD in the elderly.